Joakim Näsvall
Researcher at Institutionen för medicinsk biokemi och mikrobiologi; Infektioner och immunitet; Joakim Näsvall
- Mobile phone:
- +46 70 697 22 36
- E-mail:
- joakim.nasvall@imbim.uu.se
- Visiting address:
- BMC
Husargatan 3
752 37 UPPSALA - Postal address:
- Box 582
751 23 UPPSALA
- ORCID:
- 0000-0002-6831-3105
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Research
Evolution of new genes
Our research focuses on a very fundamental question in evolutionary biology; how new genes come into existence and how they evolve. A new gene can appear in a genome by three different routes: (A) Horizontal (lateral) gene transfer, where a gene is transferred from another organism. (B) De novo evolution, where a previously non-functional DNA sequence acquires a function. (C) Duplication-divergence, where a copy of a duplicated ancestral gene acquires a new function. Our current research mainly deals with duplication-divergence.
We use experimental evolution and genetic methods in bacterial model systems for studying how gene duplication and amplification, neutral mutations, functional trade-offs and other factors influence the appearance of new functions and their further evolution. We are also interested in other questions like evolution of the components of the translational apparatus and the genetic code.
Publications
Selection of publications
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
- Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA (2018)
- Direct and Inverted Repeat stimulated excision (DIRex) (2017)
- Duplication-Insertion Recombineering (2017)
- Evolution of New Functions De Novo and from Preexisting Genes (2015)
- Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence (2012)
- Activation of cryptic aminoglycoside resistance in Salmonella enterica (2011)
Recent publications
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Mutational Pathways and Trade-Offs Between HisA and TrpF Functions (2020)
- Selection for novel metabolic capabilities in Salmonella enterica (2019)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
All publications
Articles
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Mutational Pathways and Trade-Offs Between HisA and TrpF Functions (2020)
- Selection for novel metabolic capabilities in Salmonella enterica (2019)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
- Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA (2018)
- Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica (2018)
- Evolution of antibiotic resistance without antibiotic exposure (2017)
- Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes (2017)
- Direct and Inverted Repeat stimulated excision (DIRex) (2017)
- Duplication-Insertion Recombineering (2017)
- Compensating the fitness costs of synonymous mutations (2016)
- Evolution of New Functions De Novo and from Preexisting Genes (2015)
- Structure of AadA from Salmonella enterica (2015)
- Two-step Ligand Binding in a (βα)8 Barrel Enzyme (2015)
- Minor Fitness Costs in an Experimental Model of Horizontal Gene Transfer in Bacteria (2014)
- Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence (2012)
- Activation of cryptic aminoglycoside resistance in Salmonella enterica (2011)
- Intermittent selection directs evolution of a specialist enzyme to become a generalist
- Evolvability of orthologous genes
- Crystal structure of AadA at 2.5 Å resolution - an aminoglycoside 3" adenyltransferase
- Synonymous mutations that reduce S20 levels can be compensated by mutations in fis and rpoA
- Genetic adaptations to growth under laboratory conditions in Escherichia coli and Salmonella enterica
- Functional trade-offs during evolution of new functions in the HisA-TrpF system
- Ribosomal protein L1 is required for balanced formation of ribosomal subunits
- Experimental evolution of novel metabolic capabilities in Salmonella enterica
